About Enzymes

Discover the latest innovations in aesthetic medicine and skincare technology.

Understanding Enzymes in Modern Aesthetic Medicine

Enzymes are proteins that act as specific catalysts for various chemical reactions. They bind to their substrates at a specific site of the molecule (the “active center”) in a model known as “lock and key.” In this model, the structures of the substrate (key) and the enzyme's active center (lock) complement each other.

Recombinant Enzymes in Modern Aesthetics

All recombinant enzymes used as active ingredients in modern aesthetic treatments are produced using the latest advances in genetic engineering and are synthesized by special bacteria. This results in more selective, pure, and less immunogenic enzymes compared to peptides or proteins purified from natural sources (such as porcine insulin).

“The future of aesthetic medicine lies in the precise application of recombinant enzymes, offering targeted solutions for various skin and subcutaneous tissue pathologies.”

Watch: The Latest in Aesthetic Technology

Key Recombinant Enzymes in Aesthetic Medicine

Collagenase GH PB220

Promotes regeneration and reorganization of collagen fibers in the dermis, improves skin texture and appearance, increasing its elasticity and tone through a powerful lifting effect.

Keratinase PB333

Through selective technology, it promotes cell renewal and gently but effectively exfoliates the skin without causing breakouts. Increases skin permeability and improves penetration of other active substances.

Hyaluronidase PB3000

Breaks down excess polysaccharides responsible for fluid retention, increasing skin permeability and promoting lymphatic drainage. Helps reduce body volume, improves capillary circulation, and enhances skin appearance.

Lipase PB500

Activates lipid metabolism within fat cells, reduces excess volume and localized fat without tissue damage. Removes excess sebum at the surface level, resulting in cleaner skin with improved appearance and texture.

Lyase PB72K

Increases tissue permeability and enhances the penetration effects of collagenase and lipase. Breaks down molecules responsible for retaining fluid and toxins in the extracellular matrix, reducing body volume and improving skin appearance.

Biotechnological Active Ingredients

Modern aesthetic treatments combine recombinant enzymes with various biotechnological active ingredients for enhanced results:

  • Vitamin A: Reduces excessive sebum production, stimulates endogenous collagen production, and maintains optimal dermal hydration.
  • B-Complex Vitamins: Regulates sebum secretion, provides anti-inflammatory effects, and restores the skin's protective barrier.
  • Vitamin C: A powerful antioxidant that prevents and slows cell aging, stimulates collagen production, and reduces melanin production.
  • Hyaluronic Acid: Both low and high molecular weight forms work together to provide deep hydration and improve skin barrier function.
  • DMAE: A natural antioxidant providing instant lifting effects and improving skin tone and elasticity.

The combination of recombinant enzymes with these active ingredients represents the cutting edge of aesthetic medicine, offering targeted, effective solutions for various skin concerns while maintaining the highest safety standards.

Detailed Overview of Recombinant Enzymes

What Are “Recombinant” Enzymes?

PBserum enzymes are of bacterial origin and developed through an advanced biotechnological process patented by Proteos Biotech. The key innovation is the use of DNA recombinant technology—a method in which genes encoding desired proteins (enzymes) are inserted into a plasmid using ligase. This recombinant DNA is then introduced into bacterial microorganisms, which in large quantities produce enzymes that structurally and sequentially match human enzymes.

Since bacteria lack the Golgi apparatus and endoplasmic reticulum found in human cells, these enzymes are non-glycosylated, resulting in higher purity and reduced risk of immune reactions.

Thanks to this, treatments using recombinant enzymes demonstrate low immunogenicity, making them safe, effective, and biologically compatible. PBserum enzymes are also processed using lyophilization (freeze-drying)—a dehydration technique that involves rapid freezing and sublimation under reduced pressure. This ensures maximum purity and stability, preserving 100% of enzyme and vitamin activity without the need for preservatives or additives. Lyophilized enzymes can be stored at room temperature (25 °C ± 5 °C) without refrigeration. However, once reconstituted in saline, they must be used immediately to avoid contamination.

Each enzyme functions best at temperatures between 36–40 °C.

Which Bacteria Are Used in PBserum Enzyme Production?

PBserum enzymes are produced in Escherichia coli, which is genetically modified to transcribe specific enzymes. The source genes are derived from:

  • Streptococcus pyogenes – for hyaluronidase PB3000
  • Clostridium histolyticum – for collagenase G/H PB220
  • Thermus thermophilus – for lipase PB500

Enzyme Structure and Selectivity

PBserum products are formulated as microemulsions—oil-based solutions where tiny particles of two immiscible liquids form stable microcapsules. This allows for effective enzyme penetration and targeted delivery, improving treatment outcomes. The texture facilitates smooth application and massage without residue.

The effectiveness of PBserum recombinant enzymes lies in their high specificity and selectivity for target substrates, as well as their ability to support tissue regeneration mechanisms. Used properly, they help treat a variety of skin conditions, including aging signs, pigmentation, acne scars, fibrosis, cellulite, and localized fat.

PBserum Enzyme Overview

KERATINASE PB333

A recombinant enzyme from Bacillus licheniformis.

  • Breaks peptide and disulfide bonds in keratin
  • Promotes natural epidermal renewal
  • Increases skin permeability, enhancing absorption of other actives (by up to 9×)
  • Highly selective and safe for even sensitive skin

COLLAGENASE G/H PB220

Recombinant enzyme from Clostridium histolyticum.

  • Breaks down aged collagen fibers
  • Stimulates synthesis of new collagen
  • Remodels the collagen matrix

LIPASE PB500

A recombinant enzyme that targets adipose tissue.

  • Breaks down triglycerides into glycerol and fatty acids
  • Regulates sebum composition
  • Improves pore condition
  • Does not cause adipocyte membrane lysis (unlike deoxycholic acid), reducing inflammation risk

HYALURONIDASE PB3000

From Streptococcus pyogenes.

  • Breaks down hyaluronic acid
  • Increases tissue permeability and fluid drainage
  • Enhances penetration of other enzymes in the PBserum complex

LYASE PB72K

  • Stimulates fibroblasts
  • Increases production of collagen, elastin, and hyaluronic acid
  • Releases growth factors trapped in the matrix
  • Promotes repair and regeneration